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. 2021 Aug 30;16(8):e0250332. doi: 10.1371/journal.pone.0250332

Table 2. Model estimates for predicting blood values.

Model Variable Parameter Estimate Standard Error t p
Species: Tursiops truncatus
Lab: AHDC
i Hematocrit Intercept 44.06 0.45 97.38 < 0.001
Sex -2.31 0.68 -3.42 0.001
ii RBC Intercept 3.41 0.04 87.86 < 0.001
Sex -0.26 0.06 -4.52 < 0.001
iii Chloride Intercept 116.63 0.43 269.58 < 0.001
Sex 2.76 0.67 4.14 < 0.001
iv Platelet Count Intercept 132.46 5.36 24.72 < 0.001
Age -1.11 0.23 -4.85 < 0.001
v Creatine Kinase Intercept 162.03 8.61 18.82 < 0.001
Age -1.40 0.37 -3.83 < 0.001
vi Triglycerides Intercept 37.26 5.11 7.29 < 0.001
Age 0.79 0.22 3.56 0.001
vii Creatinine Intercept 1.40 0.07 19.29 < 0.001
Age 0.01 < 0.01 2.91 0.005
viii Alkaline Phosphatase Intercept 739.79 69.70 10.61 < 0.001
Age -31.59 6.85 -4.61 < 0.001
Age2 0.49 0.14 3.49 0.001
ix Reticulocyte Count Intercept 3.59 0.27 13.46 < 0.001
Month -0.19 0.04 -5.26 < 0.001
x Absolute Reticulocyte Count Intercept 113.80 6.49 17.54 < 0.001
Month -6.12 0.98 -6.25 < 0.001
xi Lymphocytes Intercept 1.31 0.14 9.51 < 0.001
Age -0.02 0.01 -3.14 0.003
Sex 0.44 0.13 3.43 0.001
xii MPV Intercept 13.89 0.50 27.89 < 0.001
Age 0.09 0.02 4.27 < 0.001
Month -0.11 0.03 -3.61 0.002
Species: Tursiops truncatus
Lab: UNAM
xiii Triglycerides Intercept 0.59 0.05 12.55 < 0.001
Sex 0.21 0.06 3.26 0.002
xiv Hematocrit Intercept 40.87 0.37 110.43 < 0.001
Month 0.16 0.05 3.49 0.001
xv Reticulocytes Intercept 1.86 0.13 13.96 < 0.001
Month -0.05 0.02 -2.70 0.008
xvi Monocytes Intercept 0.24 0.02 11.55 < 0.001
Month -0.01 < 0.01 -2.98 0.004
xvii RDWa Intercept 99.33 1.88 52.76 < 0.001
Month 1.15 0.26 4.47 < 0.001
xviii Pt/Fin Intercept 43.69 1.59 27.49 < 0.001
Month -0.95 0.23 -4.13 < 0.001
xix Total Protein Intercept 64.26 0.79 81.85 < 0.001
Month 0.35 0.11 3.29 0.002
xx Globulin Intercept 23.39 0.62 37.97 < 0.001
Month 0.23 0.08 2.69 0.009
xxi Phosphorus Intercept 1.27 0.03 37.67 < 0.001
Month 0.02 < 0.01 3.78 < 0.001
xxii Ca/P Ratio Intercept 1.84 0.09 21.10 < 0.001
Month -0.02 0.01 -2.91 0.007
xxiii Potassium Intercept 3.50 0.05 76.11 < 0.001
Month -0.02 0.01 -3.52 0.001
xxiv Sodium Intercept 155.07 0.66 234.42 < 0.001
Month -0.33 0.09 -3.71 < 0.001
xxv Bicarbonate Intercept 25.44 0.64 39.70 < 0.001
Month 0.37 0.10 3.83 < 0.001
xxvi Strong Ion Ratio Intercept 32.32 0.55 58.59 < 0.001
Month -0.50 0.08 -6.38 < 0.001
xxvii Amylase Intercept 2.38 0.61 3.92 < 0.001
Month 0.58 0.08 6.87 < 0.001
xxviii Lipase Intercept 29.73 2.11 14.07 < 0.001
Month -0.96 0.28 -3.41 0.001
Species: Tursiops aduncus
Lab: AHDC
xxix Cholesterol Intercept 137.36 6.60 20.80 < 0.001
Sex 31.56 9.92 3.18 0.004
xxx Triglycerides Intercept 32.69 2.40 13.61 < 0.001
Sex 13.64 3.61 3.78 0.001
xxxi Hematocrit Intercept 42.74 1.35 31.66 < 0.001
Age 0.08 0.06 1.34 0.199
xxxii Alkaline Phosphatase Intercept 1212.29 146.37 8.28 < 0.001
Age -22.58 6.45 -3.50 0.002
xxxiii MPV Intercept 12.06 < 0.01 22485.33 < 0.001
Age 0.63 < 0.01 7136.66 < 0.001
Age2 -0.02 < 0.01 -6080.57 < 0.001
xxxiv Creatinine Intercept 1.32 0.13 10.28 < 0.001
Age 0.05 0.01 3.25 0.003
Age2 < 0.01 < 0.01 -3.67 0.002
xxxv MCHC Intercept 34.78 < 0.01 45735233.10 < 0.001
Age 0.12 < 0.01 1073540.84 < 0.001
Age2 < 0.01 < 0.01 -952034.69 < 0.001
xxxvi MCV Intercept 113.91 1.09 104.34 < 0.001
Month 0.44 0.06 7.18 0.005
xxxvii Segmented Neutrophils Intercept 4.04 0.53 7.69 < 0.001
Month -0.27 0.08 -3.43 0.002
xxxviii Eosinophils Intercept 1.34 0.19 6.99 < 0.001
Month -0.13 0.03 -4.19 < 0.001
xxxix Bicarbonate Intercept 26.95 0.58 46.60 < 0.001
Month -0.48 0.09 -5.47 < 0.001
xxxx Calcium Intercept 9.62 0.11 84.52 < 0.001
Month -0.08 0.02 -4.44 < 0.001
xxxxi Total Protein Intercept 7.11 0.13 53.12 < 0.001
Month -0.11 0.02 -5.49 < 0.001
xxxxii Albumin Intercept 5.06 0.11 46.98 < 0.001
Month -0.06 0.02 -3.78 0.001
xxxxiii ALT Intercept 27.16 12.52 2.17 0.042
Month 9.03 1.89 4.77 < 0.001
xxxxiv AST Intercept 199.62 36.72 5.44 < 0.001
Month 18.30 5.01 3.66 0.004
xxxxv Lipase Intercept 4.31 0.75 5.71 < 0.001
Month 0.33 0.12 2.80 0.010
xxxxvi Lipemia Intercept 4.86 0.64 7.60 < 0.001
Month 0.42 0.10 4.24 < 0.001
xxxxvii TIBC Intercept 411.81 25.36 16.24 < 0.001
Sex 85.36 23.29 3.67 0.001
Age -5.30 1.21 -4.37 < 0.001
xxxxviii Hemoglobin Intercept 15.75 0.02 654.43 < 0.001
Sex 1.26 0.30 4.18 < 0.001
Age -0.13 0.01 -9.78 < 0.001
Age2 < 0.01 < 0.01 5.69 < 0.001
xxxxix Lymphocytes Intercept 2.08 0.28 7.33 < 0.001
Age -0.09 0.02 -3.82 0.001
Age2 < 0.01 < 0.01 3.61 0.001
Month -0.10 0.03 -3.04 0.005
xxxxx Urea Nitrogen Intercept 35.20 3.25 10.82 < 0.001
Age 1.03 0.31 3.35 0.003
Age2 -0.03 0.01 -3.93 0.001
Month 1.36 0.32 4.24 < 0.001
xxxxxi Fibrinogen Intercept 335.41 16.51 20.32 < 0.001
Sex 23.76 7.74 3.07 0.009
Age -6.68 1.38 -4.83 0.001
Age2 0.15 0.04 3.81 0.003
Month -11.08 2.17 -5.11 < 0.001
Species: Delphinapterus leucas
Lab: AHDC
xxxxxii Calcium Intercept 11.33 0.14 83.07 < 0.001
Age -0.16 0.01 -12.38 < 0.001
Age2 < 0.01 < 0.01 10.98 < 0.001
xxxxxiii Reticulocyte Count Intercept 2.15 0.26 8.39 < 0.001
Sex 0.38 0.10 3.73 0.004
Age -0.13 0.03 -4.95 0.002
Age2 < 0.01 < 0.01 4.58 0.001
xxxxxiv Absolute Reticulocyte Count Intercept 74.57 7.31 10.20 < 0.001
Sex 10.17 2.76 3.69 0.005
Age -5.01 0.75 -6.68 < 0.001
Age2 0.11 0.02 6.59 < 0.001

Mixed models included the explanatory variables found to be significant predictors. Possible fixed effects included: sex, age, age2, and month. Random effects included: animal ID and facility ID.