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. Author manuscript; available in PMC: 2021 Aug 30.
Published in final edited form as: Microrna. 2020;9(1):70–80. doi: 10.2174/2211536608666190624114424

Fig. (4).

Fig. (4).

Deletion of Exon 25 in DICER1 results in activation of mTORC1. (a) RT-PCR confirming deletion of exon 25 from DICER1 mRNA, the band in the Crispr/Cas9 cells is the correct size for transcripts lacking exon 25 (b) Western blot analysis comparing DICER1Delex25(+/−) to PTEN(−/−) cells. (c) Quantification of the western blots show significant increases in p4EBP1 and pS6Kinase and decreases in pAKT450, pAKT473 and DICER1. Black vertical bars show standard deviations. * denotes p-value <.05. (A higher resolution/colour version of this figure is available in the electronic copy of the article).