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DICER1Del25(+/−) Cells demonstrate the ability to form Oct4+ and SOX2+ spheres in culture. (a) After 72 hours of growth without serum DICER1Del25(+/−) continue to divide and form spheres which detach (b) upon reintroduction of serum the spheres attach and spread. (c) spheres are Oct4 positive (d) spheres are Sox2 positive. (A higher resolution/colour version of this figure is available in the electronic copy of the article).
