Figure 1.

LPS challenge induces Lcn2 expression. WT mice were intraperitoneally injected with saline (Control) or 2 μg/g LPS. (A) Representative confocal images of Lcn2 expression in the liver, jejunum and ileum of mice at 24 h post LPS injection (n = 4). DAPI, blue; Lcn2, green. (B–D) Protein levels of Lcn2 in indicated tissues from WT mice after LPS challenge at different time points were examined by Western blot (n = 4). Values were average means of triplicate experiments. Results were expressed as means ± SEM. Statistical analysis used Mann–Whitney U-tests. *P < 0.05, **P < 0.01.