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. Author manuscript; available in PMC: 2021 Aug 30.
Published in final edited form as: Structure. 2021 Mar 22;29(7):743–754.e4. doi: 10.1016/j.str.2021.03.004

KEY RESOURCES TABLE

REAGENT or RESOURCE SOURCE IDENTIFIER
Bacterial and Virus Strains
E. coli BL21(DE3) competent cells New England Biolabs Cat# C2527I
Chemicals, Peptides, and Recombinant Proteins
Recombinant SaBphP2, aa 1 – 489 (Sanchez et al., 2019) N/A
50% w/v PEG 4000 Hampton Research HR2-529
20% w/v Benzamidine Hydrochloride Hampton Research HR2-428-69
1M Sodium citrate tribasic dihydrate pH 5.6 Hampton Research HR2-935-15
Nuclear grade grease Super Lube 42150
Deposited Data
SaBphP2 PCM structure 5 ns after laser excitation This paper PDB: 7JR5
SaBphP2 PCM structure 33 ms after laser excitation This paper PDB: 7JRI
SaBphP2 PCM structure, reference in the dark (Sanchez et al., 2019) PDB: 6PTQ
Recombinant DNA
pET28c-SaBphP2 PCM (1–489aa) (Sanchez et al., 2019) N/A
Software and Algorithms
Data processing pipeline at SACLA (Nakane et al., 2016)
Cheetah (Barty et al., 2014) https://github.com/biochem-fan/
CrystFEL (White et al., 2016) https://github.com/biochem-fan/
Structural Modeling: Coot (Emsley et al., 2010) https://www.ccp4.ac.uk/
Structural Refinement: Refmac (Murshudov et al., 2011) https://www.ccp4.ac.uk/
Pearson Correlation Coefficient, pcc.f This paper Available from lead contact
UCSF chimera (Pettersen et al., 2004) https://www.cgl.ucsf.edu/chimera/
Other
TALON® Metal Affinity Resin Takara Bio Cat# 635503
Amicon Ultra Centrifugal Filters Millipore Sigma Cat# UFC901024