Bacterial and Virus Strains |
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E. coli BL21(DE3) competent cells |
New England Biolabs |
Cat# C2527I |
Chemicals, Peptides, and Recombinant Proteins |
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Recombinant SaBphP2, aa 1 – 489 |
(Sanchez et al., 2019) |
N/A |
50% w/v PEG 4000 |
Hampton Research |
HR2-529 |
20% w/v Benzamidine Hydrochloride |
Hampton Research |
HR2-428-69 |
1M Sodium citrate tribasic dihydrate pH 5.6 |
Hampton Research |
HR2-935-15 |
Nuclear grade grease |
Super Lube |
42150 |
Deposited Data |
|
|
SaBphP2 PCM structure 5 ns after laser excitation |
This paper |
PDB: 7JR5 |
SaBphP2 PCM structure 33 ms after laser excitation |
This paper |
PDB: 7JRI |
SaBphP2 PCM structure, reference in the dark |
(Sanchez et al., 2019) |
PDB: 6PTQ |
Recombinant DNA |
|
|
pET28c-SaBphP2 PCM (1–489aa) |
(Sanchez et al., 2019) |
N/A |
Software and Algorithms |
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Data processing pipeline at SACLA |
(Nakane et al., 2016) |
|
Cheetah |
(Barty et al., 2014) |
https://github.com/biochem-fan/
|
CrystFEL |
(White et al., 2016) |
https://github.com/biochem-fan/
|
Structural Modeling: Coot |
(Emsley et al., 2010) |
https://www.ccp4.ac.uk/
|
Structural Refinement: Refmac |
(Murshudov et al., 2011) |
https://www.ccp4.ac.uk/
|
Pearson Correlation Coefficient, pcc.f |
This paper |
Available from lead contact |
UCSF chimera |
(Pettersen et al., 2004) |
https://www.cgl.ucsf.edu/chimera/
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Other |
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|
TALON® Metal Affinity Resin |
Takara Bio |
Cat# 635503 |
Amicon Ultra Centrifugal Filters |
Millipore Sigma |
Cat# UFC901024 |