Figure 4.

AML-induced loss of erythroblasts causes iron redistribution. (A) Peripheral blood counts for white blood cells (WBC), phenotypically defined B and T cells, platelets (PLT), red blood cells (RBCs), and reticulocyte absolute numbers (Ret#). Each dot represents a mouse. (B) Flow cytometry analysis of BM Ter119⁺ and Ter119⁺CD71⁺ erythroid progenitors and of different erythropoiesis stages (I-V). n = 4 control and 3 AML mice. (C) Representative FACS plots of BM erythropoiesis in control and AML-burdened mice. (D) Flow cytometry analysis of spleen Ter119⁺ and Ter119⁺CD71⁺ erythroid progenitors and of different erythropoiesis stages (I-V). n = 4 control and 3 AML mice. (E) Representative FACS plots of BM erythropoiesis in control and AML-burdened mice. Bone marrow and splenic Erfe (F) expression in control and AML-burdened mice, as assessed by qPCR. n = 7-10 control and 9-10 AML mice wild-type B6 mice were splenectomized (Sx), transplanted (Sx-AML), or not transplanted (Sx-C) (G) with MLL-AF9⁺ AML and analyzed at full infiltration. (H) Representative FACS plots of BM and liver erythropoiesis in splenectomized mice. Peripheral blood counts for PLT (I) and for RBCs and Ret# (J) in splenectomized mice. Sx-AML had increased SI and TSAT (K) and presence of toxic NTBI (L). Each dot represents a mouse.