Figure 3.

PSF-SOD1 increases intracellular total SOD enzyme activity. VERO and HK-2 cells were treated with 1, 2, 4 μM PSF-SOD1 and 1, 2, 4 μM TAT-SOD1 for 60 min or 4 μM PSF-SOD1 and 4 μM TAT-SOD1 for 10, 30, and 60 min. (a, b) Western blot analysis with SOD1 antibody in cell lysates. (c, d) Intracellular SOD enzyme activity in cell lysates was measured by SOD assay kit. ^##p < 0.01, ^###p < 0.001 compared with control cells. ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001 compared with TAT-SOD1-treated cells.