Table 1.
Primers used for detection of aminoglycoside-modifying enzyme-encoding genes by PCR.
| Target genes | Primer sequences (5′ to 3′) | Amplicon size (bp) | Annealing temperature (°C) | References |
|---|---|---|---|---|
| ANT(3 ″ )-Ia | Pri F: TGT AGA AGT CAC CAT TGT TG | 152 | 51 | This study |
| Pri R: TCA GCA AGA TAG CCA GAT | ||||
| ANT(2 ″ )-Ia | Pri F: GCA GGT CAC ATT GAT ACA C | 225 | 54 | This study |
| Pri R: TCC GCT AAG AAT CCA TAG TC | ||||
| AAC(6′)-Ib | Pri F: GAC CAA CAG CAA CGA TTC | 375 | 57 | This study |
| Pri R: AAC AGC AAC TCA ACC AGA | ||||
| AAC(6′)-IIa | Pri F: CCA TAA CTC TTC GCC TCA T | 442 | 48 | This study |
| Pri R: AAT CCT GCC TTC TCA TAG C | ||||
| APH(3′)-IIb | Pri F: TTC GTC AAG CAG GAA GTC | 662 | 50 | This study |
| Pri R: TAG AAG AAC TCG TCC AAT AGC | ||||
| APH(3′)-Ib | Pri F: TTG TTG TTG ACG CAT TGG | 284 | 56 | This study |
| Pri R: GCC GAC TAC CTT ACC TTC | ||||
| APH(3 ″ )-Ib | Pri F: GGT GAT AAC GGC AAT TCC | 548 | 56 | This study |
| Pri R: GGT CCA ATC GCA GAT AGA |