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. 2021 Jun 24;413(20):5109–5119. doi: 10.1007/s00216-021-03473-7

Fig. 4.

Fig. 4

Chromatograms of OGNs separated on the SG-DNA column at different experimental conditions: A mobile phase composition: 0.5 M sodium chloride/0.05 M sodium citrate and water, gradient elution program: 0–5 min 100% of salt solution to 100% of water at 5.1 min, then 5.1–50 min 100% of water; B mobile phase composition 0.05 M sodium chloride/0.005 M sodium citrate and water, gradient elution program: 0 min—100% of salt solution, 20 min—0% of salt (100% of water), 20–30 min—100% of water. Other conditions: flow rate 0.3 mL/min, UV-Vis detection at λ = 260 nm, column temperature 20 °C, injection volume 2 μL