Table 2.
The impact of the mobile phase composition on retention factor values for OGNs analyzed in AC. Experimental conditions: temperature gradient from 10 to 80 °C in 5 min, next the temperature was constant at 80 °C; for other conditions, see in the “Materials and methods” section
| Oligonucleotide | k at different mobile phases | ||||||
|---|---|---|---|---|---|---|---|
| 0.025 M NaCl in 2.5 mM sodium citrate (pH = 7) | 0.05 M NaCl in 5 mM sodium citrate (pH = 7) | 0.1 M NaCl in 10 mM sodium citrate (pH = 7) | 0.15 M NaCl in 15 mM sodium citrate (pH = 7) | 0.1 M NaCl in 10 mM TRIS-HCl (pH = 7) | 0.05 M NaCl in 5 mM sodium citrate, 5 mM MgCl2 (pH = 7) | 0.05 M NaCl in 5 mM sodium citrate, 10 mM MgCl2 (pH = 7) | |
| OL1 | tM | 25.7 ± 0.6 | 43.5 ± 0.6 | Not eluted | 42.9 ± 0.7 | 35.7 ± 0.7 | 39.2 ± 0.5 |
| OL2 | tM | 17.0 ± 0.3 | 26.6 ± 0.1 | 34.6 ± 0.5 | 25.6 ± 0.1 | 22.3 ± 1.0 | 25.00 ± 0.4 |
| OL4 | tM | 14.6 ± 0.4 | 20.7 ± 0.2 | 25.2 ± 0.6 | 25.0 ± 0.3 | 17.9 ± 0.4 | 21.0 ± 0.4 |
| OL6 | tM | tM | 0.8 ± 0.08 | 1.1 ± 0.06 | 0.9 ± 0.06 | 0.6 ± 0.08 | 0.8 ± 0.07 |
| OL7 | tM | tM | 0.8 ± 0.07 | 1.0 ± 0.1 | 0.9 ± 0.04 | 0.5 ± 0.1 | 0.8 ± 0.03 |
| OL8 | tM | tM | 0.4 ± 0.04 | 0.9 ± 0.08 | 0.8 ± 0.01 | tM | 0.4 ± 0.1 |