System serology profiling of NVX-CoV2373 immunized rhesus macaques
Serum was collected day 21 and day 31/32 after the first dose of NVX-CoV2373 and was profiled for the anti-NVX-CoV2373 antibody response (n = 4–5/group).
(A and B) Luminex was used to quantify the (A) antibody isotypes (IgG1, IgA, and IgM) and (B) FcR binding (FcγRIIA-1, FcγRIIA-2, and FcγRIIIA) for the anti-NVX-CoV2373 antibody response.
(C) The functional anti-NVX-CoV2373-specific antibody responses for antibody-dependent cellular phagocytosis, antibody-dependent neutrophil phagocytosis, antibody-dependent complement deposition, and antibody-dependent NK degranulation (measured by percentage of CD107).
The bars represent the means, and the error bars indicate the SD. Individual animal values are indicated by colored symbols. A two-way ANOVA with Tukey correction for multiple comparisons was used to compare antibody levels between groups. ns, not significant, ∗p ≤ 0.05, ∗∗p ≤ 0.01, ∗∗∗p ≤ 0.001, ∗∗∗∗p ≤ 0.0001.