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. 2021 Jul 22;413(21):5439–5451. doi: 10.1007/s00216-021-03525-y

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© The Author(s) 2021

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Fig. 2 — a Relationship between retention time of the fatty acids and the number of carbon atoms as well as double bounds. b Chromatographic separation for isomeric fatty acids. Separation was carried out on an RP-8 column (2.1 × 100 mm, 2.6 μm core–shell particle, pore size 10 nm) with (a) a H₂O/ACN/MeOH/HAc gradient with a flow rate of 0.3 ml/min. The void volume was approx. 0.24 ml (0.8 min)