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. 2021 Aug 30;12(9):818. doi: 10.1038/s41419-021-04087-8

Fig. 5. Hsa_circ_0001610 weakened the radiosensitivity of EC cells by suppressing the miR-139-5p expression.

Fig. 5

Ishikawa and HEC-1B cells were transfected with vector, hsa_circ_0001610, hsa_circ_0001610 + miR-NC, or hsa_circ_0001610 + miR-139-5p mimic, followed by exposure to 4 Gy irradiation. The expression levels of A hsa_circ_0001610 and B miR-139-5p was measured by qRT-PCR. The mRNA and protein levels of cyclin B1 in C Ishikawa and D HEC-1B cells were measured by qRT-PCR and Western blot, respectively. E Cell viability was measured by MTT assay. F Cell proliferation was measured by colony formation assay. G Cell invasion was measured by Transwell assay. H Cell apoptosis was measured by flow cytometry. I Cell cycle distribution was determined by PI staining. *p < 0.05.