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. 2021 Aug 30;11:17329. doi: 10.1038/s41598-021-96841-3

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Decreased mitochondrial ROS by 950 nm NIR irradiation. (A) MFI of Mito-SOX (mitochondrial ROS) was slightly reduced. The two groups did not show a significant difference. Data are mean ± SD (n > 5). P = 0.0914 compared to control. (B) MFI of Mito-Tracker (mitochondrial mass) was significantly enhanced. (C) Typical confocal microscopic images of Mito-Tracker-stained cells. (D) Relative amount of mitochondrial ROS per unit mitochondrial mass. To compare the ROS in each treated group with that of the control group, the MFI of Mito-SOX was divided by that of the Mito-Tracker. The two groups showed a significant difference. Data are mean ± SD (n > 5). **P < 0.01 compared to controls. P values were determined by one-way ANOVA (A–D).