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. 2021 Aug 30;12(9):816. doi: 10.1038/s41419-021-04014-x

Fig. 3. BV6 synergizes with IFNγ to induce caspase-independent cell death in apoptosis-deficient PC cells.

Fig. 3

AsPc-1, BxPc-3, Capan-1, and DanG cells were treated with 5 µM BV6 and/or 3 ng/ml IFNγ for 48 h in the presence of 20 μM zVAD.fmk with or without 20 μM Nec-1s. The amount of cell death was calculated by quantifying PI uptake determined with the ImageXpress Micro XLS system. Data are presented as percentage of PI-positive cells, and mean and SD of three independent experiments performed in triplicate are shown. ***P < 0.001.