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. 2021 Aug 30;12(9):816. doi: 10.1038/s41419-021-04014-x

Fig. 7. The TNFα autocrine/paracrine loop is involved in cGAMP/BV6-induced cell death.

Fig. 7

A mRNA expression levels of TNFα were determined in the indicated cell lines subjected to 4 µg/ml 2′3′-cGAMP for 10 min in the presence of 20 μM zVAD.fmk using qRT-PCR. Data are normalized to GAPDH expression and are presented as x-fold mRNA expression compared to control. Mean and SD of three independent experiments performed in triplicate are shown. **P < 0.01, ***P < 0.001. B AsPc-1 and BxPc-3 cells were treated with 4 µg/ml 2′3′-cGAMP for 10 min and/or 5 µM BV6 for 48 h in the presence of 20 μM zVAD.fmk and in the absence or presence of 50 µg/ml Enbrel. The amount of cell death was calculated by quantifying PI uptake determined with the ImageXpress Micro XLS system. Data are presented as percentage of PI-positive cells, and mean and SD of three independent experiments performed in triplicate are shown. *P < 0.05; **P < 0.01, n.s., not significant.