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. 2021 Aug 30;11:17357. doi: 10.1038/s41598-021-96699-5

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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T-bet suppressed expression of rorc and its target genes including il17a, il17f, and il22 in CD4 + T cells. At day 10 post first immunization with CII, CD4 + T cells were isolated from the draining LNs of WT mice (n = 8) and T-bet KO mice (n = 8). The expression levels of the genes were analyzed using qRT-PCR. Data are presented as mean ± SEM. Statistical tests: Welch’s t test. (**P < 0.01, ***P < 0.001).