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. 2021 Aug 30;11:17373. doi: 10.1038/s41598-021-96859-7

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Glyoxalase-1 (Glo-1) expression and activity and glutathione levels. (a) PCR analysis for gene expression of Glo-1 in liver from lean controls (RAGE+/+LeptrDb+/+), obese (RAGE+/+LeptrDb−/−), and RAGE KO obese (RAGE−/− LeptrDb−/−) mice. (b) Western blotting analysis for Glo-1 protein levels in liver extracts. Histograms report the densitometric analysis normalized for β-actin content. (c) Glo-1 enzymatic activity detected in liver lysates. (d) Total glutathione in liver lysates. Data are means ± SEM of 4–6 animals per group. Statistical significance: *P < 0.05; **P < 0.01; ***P < 0.001.