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. 2021 Aug 30;11:17373. doi: 10.1038/s41598-021-96859-7

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Hepatic inflammation. (a) PCR analysis for gene expression of the nuclear factor erythroid 2-related factor 2 (Nrf2) in liver mRNA extracts. (b) Western blotting analysis for protein levels of Nrf2 in cytosolic and nuclear extracts of mice liver showing nuclear translocation. (c–f) Western blotting analysis for protein levels of the NLR family pyrin domain containing 3 (NLRP3) (c), active-caspase-1 (d), pro- and cleaved-intereleukin-1β (IL-1β) (e), myeloid differentiation primary response 88 (MyD88) (f) in liver protein extracts. Data are means ± SEM of 4–6 animals per group. Statistical significance: *P < 0.05; **P < 0.01; ***P < 0.001.