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. 2021 Aug 30;12(9):823. doi: 10.1038/s41419-021-04108-6

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — A Effect of mutations in the RNA polymerase III complex on neuronal necrosis. The images are samples of one chordotonal organ neuron (Ch N), which contains six sensory neurons in a cluster. Compared to the wild-type flies, the activation of neuronal necrosis resulted in Ch N loss in the AGG (Appl-Gal4/+; UAS-GluR1^Lc, tubulin-Gal80^ts/+; UAS-GFP/+) flies. The white arrows point to the swollen neurons. In the mutants under the AGG background (Appl-Gal4/+; UAS-GluR1^Lc, tubulin-Gal80^ts/+; UAS-GFP/dBrf^c07161 and Appl-Gal4/+; UAS-GluR1^Lc, tubulin-Gal80^ts/+; UAS-GFP/dPolr3c^f05999), the average number of Ch N was plotted. n = 25 chordotonal organs from five larvae. B The climbing ability of AGG flies under the mutant background same as (A). Trial n = 5, 50−60 flies were tested for each trial. C The survival and climbing ability of AG (genotype: Appl-Gal4/+; UAS-GluR1^Lc, tubulin-Gal80^ts/+; +/+) flies under the mutant or RNAi background (genotype: Appl-Gal4/+; UAS-GluR1^Lc, tubulin-Gal80^ts/Ran^G19V,Q69L;+/+ and Appl-Gal4/+; UAS-GluR1^Lc, tubulin-Gal80^ts/+; UAS-RanRNAi /+). Besides the control under the mutant background (w¹¹¹⁸), a genetic background matched RNAi control (TB00072) was also used. This line contains an insert of the transgenic vector into the defined site on chromosome 3. Trial n = 5, 50−60 flies were tested for each trial. D The survival and climbing ability of AG flies under the Ago1RNAi and Ago2RNAi background (genotype: Appl-Gal4/+; UAS-GluR1^Lc, tubulin-Gal80^ts/UAS-Ago1RNAi; +/+ and Appl-Gal4/+; UAS-GluR1^Lc, tubulin-Gal80^ts/UAS-Ago2RNAi; +/+). A genetic background matched RNAi control (TB00073) was also used. This line contains an insert of the transgenic vector into the defined site on chromosome 2. Trial n = 5, 50−60 flies were tested for each trial.