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. 2021 Aug 30;11:17362. doi: 10.1038/s41598-021-96775-w

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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En face SHG images obtained after corneas were dissected from the globe immediately after enucleation and imaged. Keratocytes (K) are shown in red, as the live cell dye Calcein AM penetrates the live keratocyte cell membrane. Note the differences is keratocyte shape in Col5a1^+/− compared to wt and the increase in stromal folds noted in the Col5a1^+/− corneas obtained with back scattered signal. Representative images of Col5a1^+/− (n = 6) and wt (n = 6) corneas.