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Dose response and kinetics of 4-OHT treatment on Tfam genotype and TFAM protein expression. Cre(+) SK-FBs were treated with the indicated doses of 4-OHT for 3 or 6 days. (A) PCR analysis of Tfam genotype. (B) Representative western blot of TFAM and β-actin protein in a Cre(+) cell line. (C) Densitometric analysis of TFAM protein expression.
