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. 2021 Aug 13;297(3):101075. doi: 10.1016/j.jbc.2021.101075

Figure 2.

Figure 2

Deleting SETD2 fragments leads to its stabilization.A and B, cartoon illustrating the N- and C-terminal truncations of SETD2 along with its known domains. C and D, western blot of whole-cell lysates probed with the depicted antibodies. Lysates of 293T cells expressing Halo-SETD2 constructs were prepared after 12 h of MG132 (10 μM) treatment. The expected bands for the target protein are indicated by arrows. E–G, western blot of whole-cell lysates probed with the indicated antibodies. Lysates of 293T cells expressing Halo-SETD2 constructs were prepared after cycloheximide (10 μM) treatment. The duration of the treatment is shown in the figure. H, cartoon illustrating the full-length SETD2 and its truncations that were used for affinity purification from 293T cells. I, heatmap showing the components of the UPS that were affinity purified with SETD2. A yellow box indicates that the UPS component was identified in mass spectrometry analysis, whereas the black box represents the UPS component was not detected. HE, high exposure; LE, low exposure; UPS, ubiquitin–proteasome system.