Figure 3.

The SETD2 N terminus can destabilize ySet2.A, B and D, cartoon illustrating the chimeric ySet2 constructs made by fusing with the segments of N terminus of SETD2. C and E, western blot of whole-cell lysates probed with the depicted antibodies. Lysates of 293T cells expressing Halo-ySet2 constructs were prepared after 12 h of MG132 (10 μM) treatment. The expected band for the target protein is depicted by arrows. F, western blot of whole-cell lysates of yeast BY4741 expressing FLAG-ySet2 constructs (using pRS416) probed with the antibodies indicated. ∗, nonspecific; HE, high exposure; LE, low exposure.