Fig. 1.

Long-term ADT reprogrammed PC cells to enable cancer cells to grow in the bone microenvironment. (A) Morphological changes in LNCaP and those treated with MDV3100 (LNCaP-MDV) when observed by light microscopy. (B, C and D) NE markers (Chromogranin A, Synaptophysin and Neuron Specific Enolase), full-length AR (AR-Full) and AR-V7 expression were determined by qPCR. (E) AR-Full (SC, N-20 antibody), AR-V7 and Synaptophysin (Syn) expression were determined by WB. (F & G) LNCaP and LNCaP-MDV (Gc to f) were grafted into the mouse bones by intratibial injection. Tumor formation (red circle) was determined (8 weeks after grafting) by small animal X-ray radiograph imaging (F), H&E and IHC staining of AR, and AR-V7 (Ga,b: LNCaP negative; Gc to f: LNCaP-MDV). Arrow in Gb: bone marrow (blue); in Gd: new bone (black), tumor cells (green). Statistical significance was determined by student's t-test. ** p <0.001.