Fig. 4.
Blocking of GRP/GRP-R signaling in combination with ADT is sufficient to control CRPC tumor growth in vivo. 22RV1 cells were placed into the right flank of male athymic nude mice by subcutaneous (s.c.) injection. After the primary tumor formation (about 2-3 weeks), the mice were treated with RC3095 (20ug/day, sc Inj) alone or in combination with ADT for two weeks. Castration (Cx) or MDV3100 (10mg/kg/day, gavage) was used to mimic ADT. Control group mice (Con) were treated with vehicle only. (A) Tumor volume was measured weekly and calculated by the formula: Volume = π/6 × W × H × L (mm3). (B) is showing the tumor volumes at the end point. Results are expressed as the mean percentage change in tumor volume; bars, ± SD. Statistical significance was determined by student's t-test. * p <0.05; ** p <0.001.