Skip to main content
. 2021 Aug 6;297(3):100987. doi: 10.1016/j.jbc.2021.100987

Figure 7.

Figure 7

HA does not bind CD147. Immunocytochemistry analysis was used to assess HA binding to CD147 in fibroblasts (A–D) and myofibroblasts (E–H). Fibroblasts were grown to approximately 50% confluence in DMEM/F12 medium containing 10% v/v FCS. Following a 48 h growth arrest period cells were treated with TGF-β1(10 ng/ml) or fresh serum-free medium and incubated at 37 °C, 5% CO2 for 72 h. Cells were then fixed in 4% paraformaldehyde and stained with HABP (A and E) (red stain) or CD147 (B and F) (Green stain). HABP/CD147 association can be observed in fibroblasts (C and D) and myofibroblasts (G and H). Scale bar for enlarged images (D and H) is equal to150 μm. Original magnification ×400.