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. 2021 Jul 30;297(3):101025. doi: 10.1016/j.jbc.2021.101025

Figure 1.

Figure 1

Repair protein complex formation of XRCC1 and polβ. Size-exclusion chromatography profiles showing the elution volumes (EV) for individual XRCC1 (A–D) and polβ (E and F) proteins are presented as in 8.9 and 15.8 ml, respectively, on the side of respective elution peaks. XRCC1/polβ interaction mutants V86R and R109A are eluted separately (G and H). XRCC1/polβ protein complexes are presented as EV: 11.2 ml for XRCC1 cancer-associated variants (I and J) and the cerebellar ataxia-related K431N mutant (K and L). Each peak fraction is analyzed on 12% SDS-polyacrylamide (w/v) gel and compared with the molecular weight marker (M: Precision Plus Protein Dual Color Standards, 10–250 kDa).