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. 2021 Jul 30;297(3):101025. doi: 10.1016/j.jbc.2021.101025

Figure 3.

Figure 3

DNA-binding kinetics of XRCC1. The real-time DNA-binding kinetics of wild-type XRCC1 to one nucleotide gap (A) and nick DNA with 3′-dG:C (B) or 3′-8-oxodG:A (C). The sensorgrams are shown for the concentrations range of XRCC1 (0–640 nM) where the DNA with a biotin label is immobilized on the streptavidin biosensors. D, table shows comparison of the equilibrium binding constants (KD) between XRCC1 wild-type and variants for gap and nick DNA with and without damaged ends. The real-time DNA-binding measurements for XRCC1 variants are presented in Figs. S6, S8, and S9.