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. 2021 Jul 30;297(3):101025. doi: 10.1016/j.jbc.2021.101025

Figure 8.

Figure 8

Ligation of nick repair intermediate by ligase IIIα in the presence of XRCC1.A, illustrations of the nick DNA substrate with preinserted 3′-dG:C, the ligation reaction intermediates with 5′-adenylate (AMP), and ligation products. B, line 1 is the negative enzyme control of the nick DNA substrate. Lanes 2 to 7 and 8 to 13 are the ligation products in the presence and absence of XRCC1, respectively, and correspond to time points of 10, 20, 30, 40, 50, and 60 s. C and D, the graphs show time-dependent changes in the amount of ligation products in the absence and presence of XRCC1 (C) and disease-associated variants (D). The data represent the average of three independent experiments ±SD. The gel images for XRCC1 variants are presented in Fig. S13. The bar graphs with individual data points are presented in Fig. S14.