Table 2.

Raw data to calculate the P_f values of DPhPC with 30 mol% cholesterol. (Upper) Data for the UL correction. (Lower) Raw data of the membrane movements. P_f is calculated from Eq. (1) such that J_v/A_mem is divided by v_w and ΔOsm multiplied by 10⁶. These data are plotted in Fig. 2.

Osmolarity		Polarization	UL corrected		Capillary	Capillary	Partial molar
of urea	I+100 /	ratio (I+100 /	Osmotic gradient		radius	cross sectional	volume of water
(mOsm/L)	I-100	I-100 - 1)	(mOsm/L)		(mm)	area (mm2)	(vw: cm3/mol)
200	1.014	0.014	195.671		0.55	0.9503	18
600	1	0.042	587.364
1000	1.073	0.073	963.48
†100 mM NaCl both sides.
Osmolarity	Experi-	Membrane	Elapsed	Membrane	Volume	Membrane	Membrane
of urea	mental	movement	time	velocity	flux	diameter	area	Jv/
(mOsm/ L)	number	distance (µm)	(sec)	(velm: µm/s)	(Jv: µm3/s)	(µm)	(A: µm2)	A (µm/s)
200	1	5.65	374	0.01511	14,355	426.4	142,714	0.1006
	2	2.47	236	0.01047	9953	352.3	97,457	0.1021
	3	2.82	456	0.00619	5887	305.7	73,376	0.0802
	4	7.42	690	0.01075	10,212	375.7	110,776	0.0922
mean		4.590	439	0.01063	10,101	365.03	106,080	0.09378
S.D.		1.362	109.9	0.002101	1996.9	28.97	16,693	0.0058
600	1	13.08	351	0.03727	35,416	394.848	122,385	0.2894
	2	34.16	690	0.0495	47,042	456.543	163,619	0.2875
	3	1.82	70	0.02595	24,665	346.863	94,446	0.2612
	4	7.27	199	0.03652	34,704	415.413	135,466	0.2562
mean		14.080	327.5	0.037311	35,456.8	403.41675	128,979	0.27358
S.D.		8.171	154.4	0.00556	5283.6	26.327127	16,593.237	0.00999
1000	1	41.30	642	0.06433	61,135	436.435	149,523	0.4089
	2	53.69	690	0.07781	73,947	457	163,946	0.451
	3	40.55	690	0.05877	55,848	399.418	125,235	0.4459
	4	53.32	690	0.07727	73,430	461.113	166,911	0.4399
mean		47.215	678	0.069546	66,090	438.4915	151,403.75	0.43643
S.D.		4.197	13.9	0.005491	5218.12	16.279723	10,985.463	0.01091