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. 2021 Aug 18;24(9):102994. doi: 10.1016/j.isci.2021.102994

Figure 5.

Figure 5

FBP17 accumulating in the surrounding normal cells plays a positive role in apical extrusion

(A) EM images of MDCK-pTR GFP-RasV12 cells surrounded by normal MDCK or MDCK FBP17-shRNA1 cells. The intercellular regions in the white boxes 1-4 are shown below at higher magnification: (1) between normal and RasV12 cells, (2) between the first and second row of normal cells, (3) between a RasV12 cell and the first row of an FBP17-knockdown cell, (4) between the first and second row of FBP17-knockdown cells. Scale bars, 5 μm; 0.5 μm (lower panels).

(B) Fluorescence images of mix cultures of MDCK-pTR GFP-RasV12 and MDCK mCherry-FBP17 cells in the absence or presence of tetracycline. X-Y images show z stack.

(C) X-Z fluorescence images of RasV12-transformed cells surrounded by normal or FBP17-knockdown cells.

(D) Effect of FBP17 knockdown in normal cells on apical extrusion.

(E) Immunofluorescence images of Filamin in mix cultures of RasV12 cells and normal or FBP17-knockdown cells. (B, C, E) Scale bars, 10 μm.

(F) Effect of FBP17 knockdown in the surrounding normal cells on Filamin accumulation. (D, F) Data are mean ± SD. ∗p < 0.05, ∗∗p < 0.01 (unpaired two-tailed Student's t-tests).