REAGENT or RESOURCE | SOURCE | IDENTIFIER |
---|---|---|
Antibodies | ||
rabbit anti-FBP17 antibody | Itoh et al. (2005) | |
rabbit anti-GST antibody | Santa Cruz Biotechnology | Cat#sc-459; RRID: AB_631586 |
rabbit anti-DsRed antibody | Clontech | Cat#632496; RRID: AB_10013483 |
rabbit anti-RFP antibody | MBL | Cat#PM005; RRID: AB_591279 |
mouse anti-GAPDH antibody | Millipore | Cat#MAB374; RRID: AB_2107445 |
rat anti-E-cadherin antibody | Life Technologies | Cat#13-1900; RRID: AB_86571 |
rabbit anti-ZO-1 antibody | Thermo Fisher Scientific | Cat#61-7300; RRID: AB_2533938 |
mouse anti-Filamin antibody | Sigma | Cat#F6682; RRID: 2106432 |
rabbit anti-phospho-Myosin light chain 2 (Thr18/Ser19) antibody | Cell Signaling Technology | Cat#95777 |
Alexa Fluor-568-conjugated phalloidin | Invitrogen | Cat#A12380 |
Alexa Fluor-647-conjugated phalloidin | Invitrogen | Cat#A22287 |
Alexa Fluor-568 donkey anti-mouse IgG (H+L) | Life Technologies | Cat#A10037; RRID:AB_2534013 |
Alexa Fluor-568 donkey anti-rat IgG (H+L) | Life Technologies | Cat#A11077; RRID:AB_2534121 |
Alexa Fluor-647 donkey anti-rabbit IgG (H+L) | Life Technologies | Cat#A31573; RRID:AB_2536183 |
20-nm gold goat anti-rabbit IgG (H+L) | British BioCell International | Cat#EMGAR20 |
Chemicals, peptides, and recombinant proteins | ||
ML141 | Tocris Bioscience | Cat#4266 |
Trolox | Cayman Chemical | Cat#10011659 |
Blasticidin | Invivogen | Cat#ant-bl-1 |
Zeocin | Invivogen | Cat#ant-zn-1 |
G418 (Geneticin) | GIBCO | Cat#10131027 |
Tetracycline | Sigma-Aldrich | Cat#T7660 |
Paraformaldehyde | Sigma-Aldrich | Cat#6148 |
Glutaraldehyde | Electron Microscopy Sciences | Cat#16020 |
osmium tetroxide | TAAB | Cat#O003 |
Critical commercial assays | ||
MycoAlert | Lonza | Cat#LT07-118 |
Hoechst 33342 | Life Technologies | Cat#H3570 |
Type I collagen | Nitta Gelatin | N/A |
Nucleofector 2b Kit L | Lonza | Cat#VACA-1005 |
Araldite502/Embed812 kit | Electron Microscopy Sciences | Cat#13940 |
LR-white resin (medium) | Nisshin EM | Cat#3962 |
Experimental models: Cell lines | ||
MDCK | Dr. W. Birchmeier | N/A |
Oligonucleotides | ||
canine FBP17-shRNA1 forward: 5′-GATCCCCGGATCGGAGAAT CAATGAATTCAAGAGATTCATTGATTCTCCGATCCTTTTTC-3′ |
This paper | N/A |
canine FBP17-shRNA1 reverse: 5′-TCGAGAAAAAGGATCGGAG AATCAATGAATCTCTTGAATTCATTGATTCTCCGATCCGGG-3′ |
This paper | N/A |
canine FBP17-shRNA2 forward: 5′-GATCCCCGGGAAATGCTTG GATGAAATTCAAGAGATTTCATCCAAGCATTTCCCTTTTTC-3′ |
This paper | N/A |
canine FBP17-shRNA2 reverse: 5′-TCGAGAAAAAGGGAAATGC TTGGATGAAATCTCTTGAATTTCATCCAAGCATTTCCCGGG-3′ |
This paper | N/A |
Software and algorithms | ||
ImageJ | NIH Image | https://imagej.nih.gov/ij/download.html |
3DMOD | Kremer et al. (1996) | https://bio3d.colorado.edu/imod/ |