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. 2021 Aug 18;24(9):102994. doi: 10.1016/j.isci.2021.102994
REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies

rabbit anti-FBP17 antibody Itoh et al. (2005)
rabbit anti-GST antibody Santa Cruz Biotechnology Cat#sc-459; RRID: AB_631586
rabbit anti-DsRed antibody Clontech Cat#632496; RRID: AB_10013483
rabbit anti-RFP antibody MBL Cat#PM005; RRID: AB_591279
mouse anti-GAPDH antibody Millipore Cat#MAB374; RRID: AB_2107445
rat anti-E-cadherin antibody Life Technologies Cat#13-1900; RRID: AB_86571
rabbit anti-ZO-1 antibody Thermo Fisher Scientific Cat#61-7300; RRID: AB_2533938
mouse anti-Filamin antibody Sigma Cat#F6682; RRID: 2106432
rabbit anti-phospho-Myosin light chain 2 (Thr18/Ser19) antibody Cell Signaling Technology Cat#95777
Alexa Fluor-568-conjugated phalloidin Invitrogen Cat#A12380
Alexa Fluor-647-conjugated phalloidin Invitrogen Cat#A22287
Alexa Fluor-568 donkey anti-mouse IgG (H+L) Life Technologies Cat#A10037; RRID:AB_2534013
Alexa Fluor-568 donkey anti-rat IgG (H+L) Life Technologies Cat#A11077; RRID:AB_2534121
Alexa Fluor-647 donkey anti-rabbit IgG (H+L) Life Technologies Cat#A31573; RRID:AB_2536183
20-nm gold goat anti-rabbit IgG (H+L) British BioCell International Cat#EMGAR20

Chemicals, peptides, and recombinant proteins

ML141 Tocris Bioscience Cat#4266
Trolox Cayman Chemical Cat#10011659
Blasticidin Invivogen Cat#ant-bl-1
Zeocin Invivogen Cat#ant-zn-1
G418 (Geneticin) GIBCO Cat#10131027
Tetracycline Sigma-Aldrich Cat#T7660
Paraformaldehyde Sigma-Aldrich Cat#6148
Glutaraldehyde Electron Microscopy Sciences Cat#16020
osmium tetroxide TAAB Cat#O003

Critical commercial assays

MycoAlert Lonza Cat#LT07-118
Hoechst 33342 Life Technologies Cat#H3570
Type I collagen Nitta Gelatin N/A
Nucleofector 2b Kit L Lonza Cat#VACA-1005
Araldite502/Embed812 kit Electron Microscopy Sciences Cat#13940
LR-white resin (medium) Nisshin EM Cat#3962

Experimental models: Cell lines

MDCK Dr. W. Birchmeier N/A

Oligonucleotides

canine FBP17-shRNA1 forward: 5′-GATCCCCGGATCGGAGAAT
CAATGAATTCAAGAGATTCATTGATTCTCCGATCCTTTTTC-3′
This paper N/A
canine FBP17-shRNA1 reverse: 5′-TCGAGAAAAAGGATCGGAG
AATCAATGAATCTCTTGAATTCATTGATTCTCCGATCCGGG-3′
This paper N/A
canine FBP17-shRNA2 forward: 5′-GATCCCCGGGAAATGCTTG
GATGAAATTCAAGAGATTTCATCCAAGCATTTCCCTTTTTC-3′
This paper N/A
canine FBP17-shRNA2 reverse: 5′-TCGAGAAAAAGGGAAATGC
TTGGATGAAATCTCTTGAATTTCATCCAAGCATTTCCCGGG-3′
This paper N/A

Software and algorithms

ImageJ NIH Image https://imagej.nih.gov/ij/download.html
3DMOD Kremer et al. (1996) https://bio3d.colorado.edu/imod/