Fig. 3.

Pesticide treatment increases the phospho-elF2α levels in Neuro2A cells: Representative immunoblots (lower panel) showing the relative levels of total and phosphorylated (Ser-51) forms of elF2α in cells treated with indicated chemicals, and the cells were arrested at 0 or 2 h of recovery (R). Vehicle treated cells served as control and tubulin as the loading control. The bar diagram (upper panel) total and phosphorylated (Ser-51) forms of elF2α as measured by densitometric quantification of signal intensities in the immunoblots. Signal intensities were normalized for tubulin, and the values obtained for the vehicle-treated group was considered as 1. All data show mean ± s.d. (n = 3). *P < 0.05 (two-tailed, unpaired Student's t-test).