Skip to main content
. 2021 Jul 31;297(3):101031. doi: 10.1016/j.jbc.2021.101031

Figure 2.

Figure 2

Thermal shift of the NS3-4A protease harboring NS3-Q80K and epistatic amino acid substitutions. Data from real-time thermal stability assay using Sypro Orange, a temperature-stable fluorophore that exhibit enhanced fluorescence upon interacting with unfolded proteins. A, thermal stability of the WT protease and the NS3-Q80K mutant, as well as (BF), mutants harboring epistatic secondary substitutions assessed under increasing incubation temperatures. Graphs showing impact of mutations on protease protein unfolding patterns characterized by fluorescence emission curves.