FIG. 3.

Overexpression of RPB7 prevents the accelerated death of rpb4Δ cells during starvation in stationary phase. AS1 (rpb4Δ strain overexpressing RPB7) cells were grown at 28°C on SC medium lacking uracyl and containing 2% galactose. SUB62 (wild-type) and MC11-1 (rpb4Δ) cells were grown in the same medium supplemented with uracyl. The viability was determined, at various time points after entry into stationary phase, by evaluating the plating efficiency on YPG at 25°C. Symbols: ■, SUB62; ⧫, MC11-1; ▴, AS1. The graph represents an average of three different experiments. The deviation was less than 25%.