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. 2021 Jul 31;297(3):101039. doi: 10.1016/j.jbc.2021.101039

Figure 4.

Figure 4

TTR double mutants chemical equilibrium denaturation.1.5 μM of T119M-TTR or double mutants R34G/T119M and K35T/T119M TTR were incubated for 96 h at 25 °C in 50 mM sodium phosphate (pH 7.4), 0.1 M KCl with increasing urea or Gdm.HCl concentrations prior measurements of tryptophan fluorescence emission spectra. A, comparison of urea equilibrium denaturation curves of R34G with R34G/T119M TTR (upper panel) and K35 with K35T/T119 TTR (lower panel). B, urea denaturation curves of T119M, K35T/T119M and R34G/T119M. C, Gdm.HCl denaturation curves of T119M, K35T/T119M, and R34G/T119M.