Table 2.
Percentage of species determined by analytical ultracentrifugation after 96 h incubation in 50 mM sodium phosphate (pH 7.4), 0.1 M KCl, and 2.5 M urea
| 2.5 M urea | Monomer |
Tetramer |
Higher-order oligomers |
|||
|---|---|---|---|---|---|---|
| 15 μM | 5 μM | 15 μM | 5 μM | 15 μM | 5 μM | |
| WT-TTR | 0.0% | 0.0% | 95.7% | 100.0% | 4.3% | 0.0% |
| L55P-TTR | 10.4% | 16.6% | 50.8% | 44.4% | 38.8% | 39.0% |
| R34T-TTR | 3.2% | 8.1% | 81.9% | 79.5% | 14.9% | 12.4% |
| K35N-TTR | 9.2% | 15.9% | 63.7% | 57.9% | 27.1% | 26.2% |
| K35T-TTR | 12.2% | 20.1% | 46.0% | 43.7% | 41.8% | 36.2% |
| R34G-TTR | 21.0% | 29.3% | 59.5% | 59.9% | 19.5% | 10.8% |
| V30M-TTR | 15.2% | 33.7% | 59.5% | 54.3% | 25.3% | 12.0% |
| T119M-TTR | 0.0% | 0.0% | 94.8% | 100.0% | 2.5% | 0.0% |