Table 2.
Primer sequences for monkey genes examined by quantitative RT-PCR
| Gene | Primer sequences (5′ to 3′) | Product size (bp) |
|---|---|---|
| FN1 | F: ACAAGCGTGTCTCTCTGCC R: CCAGGGTGATGCTTGGAGAA |
149 |
| COL1A1 | F: CAAGGTGTTGTGCGATGACG R: CTCCCTTGGGTCCCTCGAC |
149 |
| COL4A1 | F: TTTGGCTCGCCACCATAGAG R: TCATACAGACTTGGCAGCGG |
108 |
| ACTA2a | F: GTGTTGCCCCTGAAGAGCAT R: GCTGGGACATTGAAAGTCTCA |
109 |
| PTEN | F: TGGCGGAACTTGCAATCCTCA R: TTGTCTTCCCGTCGTGTGGG |
90 |
| PDPK1 | F: GGCTCGAGAACTGGCAACCT R: GCGACATGACATCCCGCTCT |
113 |
| PIK3CA | F: AATCCCAGGTGGAATGAATGGC R: GCACCCTTTCGGCCTTTAACA |
107 |
| PHLPP1 | F: CGACAAGTCTCCAAGGTTGC R: AAGAGGTTGGCAGGCAGATG |
86 |
| BMP7 | F: AGCTTCGTCAACCTCGTGGAA R: TTGTCGAAGCGTTCCCGGAT |
158 |
| GAPDH | F: TCGTCATCAATGGAAGCCCC R: AAATGAGCCCCAGCCTTCTC |
136 |
Abbreviations: ACTA2, actin alpha 2 (smooth muscle); BMP7, bone morphogenic protein 7; COL1A1, collagen type I alpha 1 chain; COL4A1, collagen type IV alpha 1 chain; FN1, fibronectin 1; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; PDPK1, 3-phosphoinositide-dependent protein kinase 1; PHLPP1, PH domain and leucine-rich repeat protein phosphatase 1; PIK3CA, phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha; PTEN, phosphatase and tensin homolog.
a
Monkey ACTA2 uses the same primer as human.