Figure 4.
METTL3's regulation of PGC-1α is dependent on YTHDF2-based recognition of m6A. A, PGC1-α mRNA collected from an RNA immunoprecipitation assay with THP-1 cells using an IgG antibody or an anti-YTHDF2 antibody (n = 3). B, qRT-PCR determination of mRNA levels of PGC-1α and METTL3 in control and YTHDF2 knockdown cells was detected by qRT-PCR (n = 3). C, immunoblot detection of various protein levels in control and YTHDF2 knockdown cells lines, with GAPDH as a loading control (n = 3). D and E, half-lives of PGC-1α mRNA and 12S rRNA in control and YTHDF2 knockdown cells, which were used to determine half-life (n = 3). Data are represented as mean ± SD. ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001; ∗∗∗∗p < 0.0001.