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. 2021 Aug 31;20:58. doi: 10.1186/s12941-021-00467-2

Table 1.

Primers, product sizes, and annealing temperatures used for this study

Primer name Primer sequence (5´ → 3´) Amplicon size (bp) Annealing temperature (ºC) Refs.
iucA

F: AATCAATGGCTATTCCCGCTG

R: CGCTTCACTTCTTTCACTGACAGG

239 62 [18]
peg-344

F: GCGGGAAAGGACAGAAAGCCAGTG

R: GAGGGAAGATGAGAAATACGAGC

332 56 This study
iutA

F: GCCGCTAGGTTGGTGATGT

R: CTCTGGTCGTGCTGGTTGA

949 61 This study
iroB

F: GTGTTGGATTCCGCCAGTGA

R: TTCCGCCGCTACCTCTTCA

366 61 This study
magA

F: GGTGCTCTTTACATCATTGC

R: GCAATGGCCATTTGCGTTAG

1282 51 [47]
rmpA

F: GAGTATTGGTTGACAGCAGGAT

R: AGCCGTGGATAATGGTTTACAA

250 53 This study
kfu

F: ATAGTAGGCGAGCACCGAGA

R: AGAACCTTCCTCGCTGAACA

520 60 [48]
allS

F: CCGAAACATTACGCACCTTT

R: ATCACGAAGAGCCAGGTCAC

508 60 [48]
ybt

F: GACGGAAACAGCACGGTAAA

R: GAGCATAATAAGGCGAAAGA

242 60 [47]
blaCTX-M-15

F: CGCTTTGCGATGTGCAG

R: ACCGCGATATCGTTGGT

590 53 This study
blaTEM

F: GAGTATTCAACATTTCCGTGTC

R: TAATCAGTGAGGCACCTATCTC

800 54 [49]
blaSHV

F: AAGATCCACTATCGCCAGCAG

R: ATTCAGTTCCGTTTCCCAGCGG

200 60 [49]
blaOXA-48

F-GCGTGGTTAAGGATGAACAC

R-CATCAAGTTCAACCCAACCG

745 60 [49]
blaKPC-1

F: CGTCTAGTTCTGCTGTCTTG

R:CTTGTCATCCTTGTTAGGCG

798 55 [50]
blaNDM-1

F: GGTTTGGCGATCTGGTTTTC

R: CGGAATGGCTCATCACGATC

621 54 [50]
ompk36 group A

F: GAAGGCGCTCTGTCTCCTA

R: TGCCATCATAGATGTCATAGG

97 60 [13]
ompk36 group B

F: CGGTCGTGGCGCGCAGAAA

R: GGTTGTTCTGA TCGTCGGTA

125 64 [13]
ompk36 group C

F: CAACAACGGTCGT GGTTGGA

R: CCCAGTGCCGGAACACTATT

144 62 [13]
ompk36 group D

F: GAAGGTACTTCTCCGACCAA

R: AATCAGATTCTCCGTTGCCG

283 62 [13]