Fig. 1.

Up-regulation of CCL7 in hepatocytes accompanies macrophage infiltration in the murine livers. (A-C) C57/BL6 mice were injected with LPS or saline as described in the Methods. Macrophages were stained with anti-CD68. CCL7 expression was examined by qPCR and ELISA. (D-F) C57/BL6 mice were fed an MCD diet or a control diet as described in the Methods. Macrophages were stained with anti-CD68. CCL7 expression was examined by qPCR and ELISA. (G) C57/BL6 mice were injected with LPS or saline as described in Methods. Primary hepatocytes, hepatic stellate cells, and Kupffer cells were isolated and CCL7 expression was examined by qPCR. N = 5 mice for each group. (H) C57/BL6 mice were fed an MCD diet or a control diet as described in Methods. Primary hepatocytes, hepatic stellate cells, and Kupffer cells were isolated and CCL7 expression was examined by qPCR. N = 5 mice for each group. Scale bar, 100 μm.