Fig. 2.

Hepatocyte-specific BRG1 deficiency represses CCL7 expression and dampens macrophage infiltration. (A-C) BRG1 conditional knockout (LKO) mice and wild type littermates were injected with LPS for 12 h. CCL7 expression was examined by qPCR and ELISA. Macrophages were stained with anti-CD68. (D-F) BRG1 conditional knockout (LKO) mice and wild type littermates were fed an MCD diet for 4 weeks. CCL7 expression was examined by qPCR and ELISA. Macrophages were stained with anti-CD68. (G, H) HepG2 cells were transfected with siRNA targeting BRG1 or scrambled siRNA (SCR) followed by treatment with LPS for 6 h. CCL7 expression was examined by qPCR and ELISA. (I, J) HepG2 cells were transfected with siRNA targeting BRG1 or scrambled siRNA (SCR) followed by treatment with palmitate for 12 h. CCL7 expression was examined by qPCR and ELISA. (K, L) Primary hepatocytes were isolated from WT or LKO mice and treated with or without LPS for 6 h. CCL7 expression was examined by qPCR and ELISA. (M, N) Primary hepatocytes were isolated from WT or LKO mice and treated with palmitate for 12 h. CCL7 expression was examined by qPCR and ELISA.