Fig. 4.

BRG1-mediated CCL7 induction and macrophage infiltration is redox-sensitive. (A-C) BRG1 conditional knock-in (LKI) mice and wild type littermates were injected with LPS for 12 h with or without NAC. CCL7 expression was examined by qPCR and ELISA. Macrophages were stained with anti-CD68. (D-F) BRG1 conditional knock-in (LKI) mice and wild type littermates were fed an MCD diet for 4 weeks with or without NAC. CCL7 expression was examined by qPCR and ELISA. Macrophages were stained with anti-CD68. (G-I) Primary hepatocytes were infected with BRG1 adenovirus (Ad-BRG1) or the control adenovirus (Ad-GFP) followed by treatment with LPS and/or NAC. CCL7 expression was examined by qPCR and ELISA. Conditioned media were collected and chemotaxis assay was performed as described in Methods. (J-L) Primary hepatocytes were infected with BRG1 adenovirus (Ad-BRG1) or the control adenovirus (Ad-GFP) followed by treatment with palmitate and/or NAC. CCL7 expression was examined by qPCR and ELISA. Conditioned media were collected and chemotaxis assay was performed as described in Methods. Scale bar, 100 μm.