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. 2021 Aug 3;12(4):e01368-21. doi: 10.1128/mBio.01368-21

TABLE 2.

Kinetics of GH145 enzymes and sulfatases

Enzyme or sulfatase	Substrate	K_cat/K_m
GH145 enzymes
BT3686 WT^a	Wi-AGP	1.80 × 10² ± 5.6 × 10¹^b
BT3686 R333A	Wi-AGP	Inactive
BT3686 H90A	Wi-AGP	Inactive
BT3686 Y143F	Wi-AGP	Inactive
BACPLE00338 WT^a	Wi-AGP	3.57 × 10³ ± 1.05 × 10²^b
BACCELL00856 WT	Wi-AGP	1.18 × 10³ ± 6.90 × 10¹^b
BACCELL00856 H90Q	Wi-AGP	Inactive
Sulfatases
BACPLE00986 S76C	Wi-AGP	0.35 ± 0.04^b
BACPLE00986 S76C	SW-AGP	1.38 ± 0.14^b
BACPLE00986 S76C	4NP-sulfate^a	0.1447 ± 0.004^c

^aWT, wild-type enzyme; 4NP-sulfate, 4-nitrophenyl sulfate.

^bUnits are ml/mg⁻¹ min⁻¹.

^cUnit is mM⁻¹ min⁻¹.