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Unrooted phylogenetic tree of ChRs. The nodes are color coded as follows: red, confirmed anion selectivity; blue, confirmed cation selectivity; gray, nonfunctional; black, ion selectivity not determined. Thicker nodes show ChRs characterized in this study. Gray circles show ultrafast bootstrap support values above 95%. A tree file in the Newick format is available as Data Set S2, and the corresponding protein alignment is presented in Data Set S3.
