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. 2021 Jul 6;12(4):e00972-21. doi: 10.1128/mBio.00972-21

FIG 11.

FIG 11

VP24 induces translocation and degradation of BAF. (A) Immunofluorescence staining using anti-BAF antibody in HeLa cells plated in 24-well plates and transfected with 0.3 μg of GFP or HA-VP24. Chromosomes were stained with DAPI. Arrowheads indicate cells expressing VP24. (B) Immunofluorescence staining using anti-BAF and anti-VP24 antibodies of cells infected with EBOV. (C) Western blotting of HeLa cells plated in 24-well plates and transfected with 0.3 μg of pcDNA or HA-VP24 using anti-BAF antibody. Three biological replicates are shown. (D) Western blotting of A549 cells plated in 24-well plates and transfected with 0.3 μg of pcDNA or HA-VP24 using anti-BAF antibody. (E) Western blotting of HeLa cells plated in 24-well plates and transfected with 0.3 μg of pcDNA or expressing different levels of HA-VP24 (top) or GFP (bottom) using anti-BAF antibody. (F) Western blotting of A549 cells stably transfected with shluc or shlamin plated in 24-well plates and transfected with 0.3 μg of GFP or HA-VP24 using anti-BAF antibody (left). Western blotting of HeLa cells stably transfected with shluc or shlamin plated in 24-well plates and transfected with 0.3 μg of GFP or HA-VP24 using anti-BAF antibody (right).