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. 2021 Jul 6;12(4):e00972-21. doi: 10.1128/mBio.00972-21

FIG 5.

FIG 5

VP24 associates with emerin in the presence of other EBOV proteins. (A) Coimmunoprecipitation between VP24 and emerin in BSR-T7 cells cotransfected with the plasmids from the EBOV minigenome, NP, L, VP30, and VP35 (strain Makona), and in the presence or absence of HA-VP24. Expression of VP35, NP, and HA-VP24 was confirmed by Western blotting with the indicated antibodies. Expression of VP30 and L protein was verified by reverse-transcription-PCR (RT-PCR). Coimmunoprecipitation of both VP35 and NP with HA-VP24 was also confirmed; IP, immunoprecipitated samples; IPT, input cell extract. (B) Coimmunoprecipitation between VP24 and emerin in BSR-T7 cells cotransfected with HA-VP24 and pcDNA or the plasmids from the EBOV minigenome NP, L, VP30, and VP35 (strain Makona) (4 EBOV-P). Expression of VP35, NP, and HA-VP24 was confirmed by Western blotting with the indicated antibodies. Expression of VP30 and L protein was verified by RT-PCR; IP, immunoprecipitated samples; IPT, input cell extract. (C) Colocalization of HA-VP24 and emerin in Vero cells cotransfected with HA-VP24 and NP. Emerin, NP, and HA-VP24 localization are shown. Chromosomes were stained with DAPI. (D) Colocalization of VP24 and emerin in Vero cells infected with EBOV. Emerin and VP24 localization are shown. Chromosomes were stained with DAPI. Images 1 and 2 show higher magnification views of the indicated boxed areas.