TABLE 2.
Model refinement and validation statisticsa
| Rep (ssDNA) | Rep (dsDNA) | |
|---|---|---|
| Particles used for reconstruction | 169,830 | 43,282 |
| Box size | 256 × 256 | 256 × 256 |
| Pixel size | 0.832 | 0.832 |
| FSC resolution (Å)b | 3.7 | 4.0 |
| Global resolution (Å)c | 3.8 | 4.4 |
| B-factor sharpening (Å2)d | −207.1 | −250.0 |
| CC (model to map fit) | 80.8 | 75.2 |
| Model composition | ||
| Nonhydrogen atoms | 17,553 | 18,067 |
| Residues | ||
| DNA | 6 | 24 |
| Protein | 1,092 | 1,092 |
| B-factor avg (nucleotide) | 58.53 | 157.81 |
| B-factor avg (protein) | 65.21 | 81.50 |
| Root mean square deviations | ||
| Bond lengths (Å) | 0.010 | 0.009 |
| Bond angles (°) | 1.51 | 1.38 |
| Chirality (°) | 0.06 | 0.06 |
| Planarity (°) | 0.008 | 0.008 |
| Validation | ||
| Clashscore | 4.58 | 4.65 |
| Protein validation | ||
| MolProbity score | 1.89 | 1.91 |
| EMringer score | 2.85 | 2.29 |
| Ramachandran plot (%) | ||
| Favored | 98.53 | 98.53 |
| Allowed | 1.47 | 1.47 |
| Outliers | 0 | 0 |
| Rotamer outliers (%) | 7.44 | 7.55 |
| PDB entry | 7LAR | 7LAS |
| EMDB | 23249 | 23250 |
a
The difference in FSC and global resolution (as determined by 3DFSC) indicates minimal anisotropy. The refinement statistics are indicative of a structure with appropriate geometry.
b
Fourier shell correlation reported by cryoSPARC V2 using the gold standard method at a correlation coefficient of 0.143.
c
Determined by 3DFSC.
d
The B-factor sharpening applied by cryoSPARC V2 during the postrefinement process.