FIG 5.

Disruption of the LXCXE binding cleft in RB1 does not influence MmuPV1’s ability to cause papillomas in vivo. Sites on the ears of both wild-type and RB1^L mutant FVB/N mice were scarified and infected with 10⁸ VGE of MmuPV1. Mice were treated with 300 mJ UVB the next day and then monitored for papilloma formation over 4 months. (A) MmuPV1 induced warts in wild-type and RB1^L mutant FVB/N mice with a similar incidence (Fisher’s exact test, P  = 1, two-sided). Warts arising in wild-type and RB1^L mutant FVB/N mice share similar microscopic features. (B) Warts from both mouse genotypes were harvested, serially sectioned, and stained with hematoxylin and eosin (H&E), processed to detect Ki67 (Ki67, red; DAPI, blue) by immunofluorescence, and MCM7 by immunohistochemistry.